[HTML][HTML] Mutation of actin Tyr-53 alters the conformations of the DNase I-binding loop and the nucleotide-binding cleft
X Liu, S Shu, MSS Hong, B Yu, ED Korn - Journal of biological chemistry, 2010 - ASBMB
All but 11 of the 323 known actin sequences have Tyr at position 53, and the 11 exceptions
have the conservative substitution Phe, which raises the following questions. What is the
critical role (s) of Tyr-53, and, if it can be replaced by Phe, why has this happened so
infrequently? We compared the properties of purified endogenous Dictyostelium actin and
mutant constructs with Tyr-53 replaced by Phe, Ala, Glu, Trp, and Leu. The Y53F mutant did
not differ significantly from endogenous actin in any of the properties assayed, but the Y53A …
have the conservative substitution Phe, which raises the following questions. What is the
critical role (s) of Tyr-53, and, if it can be replaced by Phe, why has this happened so
infrequently? We compared the properties of purified endogenous Dictyostelium actin and
mutant constructs with Tyr-53 replaced by Phe, Ala, Glu, Trp, and Leu. The Y53F mutant did
not differ significantly from endogenous actin in any of the properties assayed, but the Y53A …
