Identification of glycoproteins on nitrocellulose membranes and gels
DJ Thornton, I Carlstedt, JK Sheehan - Molecular biotechnology, 1996 - Springer
DJ Thornton, I Carlstedt, JK Sheehan
Molecular biotechnology, 1996•SpringerIn this article we describe a procedure for the detection of glycoproteins on gels employing
the periodic acid-Schiff's reagent. In addition, a number of staining protocols and direct
binding ELISA, employing antibodies and lectins, are described for the identification and
quantitation of glycoproteins after their immobilization by dot, slot, or Western blotting onto
nitrocellulose membranes. We document, in detail, the conditions (ie, the effect of solvent
and detergents) for the immobilization of one specific family of O-linked glycoproteins …
the periodic acid-Schiff's reagent. In addition, a number of staining protocols and direct
binding ELISA, employing antibodies and lectins, are described for the identification and
quantitation of glycoproteins after their immobilization by dot, slot, or Western blotting onto
nitrocellulose membranes. We document, in detail, the conditions (ie, the effect of solvent
and detergents) for the immobilization of one specific family of O-linked glycoproteins …
Abstract
In this article we describe a procedure for the detection of glycoproteins on gels employing the periodic acid-Schiff’s reagent. In addition, a number of staining protocols and direct binding ELISA, employing antibodies and lectins, are described for the identification and quantitation of glycoproteins after their immobilization by dot, slot, or Western blotting onto nitrocellulose membranes. We document, in detail, the conditions (i.e., the effect of solvent and detergents) for the immobilization of one specific family of O-linked glycoproteins, namely mucins. However, taking into account our suggestions, these procedures should be applicable to other types of glycoprotein.
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