Isolation of rat and mouse alveolar type II epithelial cells
NL Jansing, J McClendon, H Kage, M Sunohara… - Lung Innate Immunity …, 2018 - Springer
NL Jansing, J McClendon, H Kage, M Sunohara, JR Alvarez, Z Borok, RL Zemans
Lung Innate Immunity and Inflammation: Methods and Protocols, 2018•SpringerThe gas exchange surface of the lungs is lined by an epithelium consisting of alveolar type
(AT) I and ATII cells. ATII cells function to produce surfactant, play a role in host defense and
fluid and ion transport, and serve as progenitors. ATI cells are important for gas exchange
and fluid and ion transport. Our understanding of the biology of these cells depends on the
investigation of isolated cells. Here, we present methods for the isolation of mouse and rat
ATII cells.
(AT) I and ATII cells. ATII cells function to produce surfactant, play a role in host defense and
fluid and ion transport, and serve as progenitors. ATI cells are important for gas exchange
and fluid and ion transport. Our understanding of the biology of these cells depends on the
investigation of isolated cells. Here, we present methods for the isolation of mouse and rat
ATII cells.
Abstract
The gas exchange surface of the lungs is lined by an epithelium consisting of alveolar type (AT) I and ATII cells. ATII cells function to produce surfactant, play a role in host defense and fluid and ion transport, and serve as progenitors. ATI cells are important for gas exchange and fluid and ion transport. Our understanding of the biology of these cells depends on the investigation of isolated cells. Here, we present methods for the isolation of mouse and rat ATII cells.
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