Division of giant mitochondria during recovery from cuprizone intoxication

B Tandler, CL Hoppel - The Journal of cell biology, 1973 - ncbi.nlm.nih.gov
B Tandler, CL Hoppel
The Journal of cell biology, 1973ncbi.nlm.nih.gov
MATERIALS AND METHODS Weanling male CFW Webster mice (Carworth Div. of Becton,
Dickerson and Co., New City, NY) were placed on a powdered complete diet containing
0.5% cuprizone (biscyclohexanone oxaldihydrazone)(G. Frederick Smith Chemical Go.,
Columbus, Ohio). After 9-14 days, depending on the clinical appearance of the animals, the
cuprizone-containlng diet was replaced by Purina lab chow (Ralston Purina Go., St. Louis,
Mo.). Animals were sacrificed by decapitation at various intervals after the normal diet was …
MATERIALS AND METHODS Weanling male CFW Webster mice (Carworth Div. of Becton, Dickerson and Co., New City, NY) were placed on a powdered complete diet containing 0.5% cuprizone (biscyclohexanone oxaldihydrazone)(G. Frederick Smith Chemical Go., Columbus, Ohio). After 9-14 days, depending on the clinical appearance of the animals, the cuprizone-containlng diet was replaced by Purina lab chow (Ralston Purina Go., St. Louis, Mo.). Animals were sacrificed by decapitation at various intervals after the normal diet was instituted (Table I). Specimens of liver were fixed in 2% OsO4 buffered with phosphate (20) or collidine (5) After rinsing in saline, the tissue blocks were soaked in 0.5% uranyl acetate made up in saline. The blocks were again rinsed in saline, dehydrated in graded ethanol, and embedded in Maraglas-DER 732 (Polysciences, Inc, Rydal, Pa.)(8). Thin sections were stained with uranyl acetate (26) and lead tartrate (21), and examined in a Siemens Elmiskop Ia electron microscope.
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