[HTML][HTML] Lysyl 5-hydroxylation, a novel histone modification, by Jumonji domain containing 6 (JMJD6)
JMJD6 is reported to hydroxylate lysyl residues of a splicing factor, U2AF65. In this study, we
found that JMJD6 hydroxylates histone lysyl residues. In vitro experiments showed that
JMJD6 has a binding affinity to histone proteins and hydroxylates multiple lysyl residues of
histone H3 and H4 tails. Using JMJD6 knock-out mouse embryos, we revealed that JMJD6
hydroxylates lysyl residues of histones H2A/H2B and H3/H4 in vivo by amino acid
composition analysis. 5-Hydroxylysine was detected at the highest level in histones purified …
found that JMJD6 hydroxylates histone lysyl residues. In vitro experiments showed that
JMJD6 has a binding affinity to histone proteins and hydroxylates multiple lysyl residues of
histone H3 and H4 tails. Using JMJD6 knock-out mouse embryos, we revealed that JMJD6
hydroxylates lysyl residues of histones H2A/H2B and H3/H4 in vivo by amino acid
composition analysis. 5-Hydroxylysine was detected at the highest level in histones purified …