[HTML][HTML] Phosphorylation of the cAMP-dependent protein kinase (PKA) regulatory subunit modulates PKA-AKAP interaction, substrate phosphorylation, and calcium …

S Manni, JH Mauban, CW Ward, M Bond - Journal of Biological Chemistry, 2008 - ASBMB
Subcellular compartmentalization of the cAMP-dependent protein kinase (PKA) by protein
kinase A-anchoring proteins (AKAPs) facilitates local protein phosphorylation. However, little
is known about how PKA targeting to AKAPs is regulated in the intact cell. PKA binds to an
amphipathic helical region of AKAPs via an N-terminal domain of the regulatory subunit. In
vitro studies showed that autophosphorylation of type II regulatory subunit (RII) can alter its
affinity for AKAPs and the catalytic subunit (PKA cat). We now investigate whether …