A more efficient method to generate integration-free human iPS cells
K Okita, Y Matsumura, Y Sato, A Okada, A Morizane… - Nature …, 2011 - nature.com
K Okita, Y Matsumura, Y Sato, A Okada, A Morizane, S Okamoto, H Hong, M Nakagawa…
Nature methods, 2011•nature.comWe report a simple method, using p53 suppression and nontransforming L-Myc, to generate
human induced pluripotent stem cells (iPSCs) with episomal plasmid vectors. We generated
human iPSCs from multiple donors, including two putative human leukocyte antigen (HLA)-
homozygous donors who match∼ 20% of the Japanese population at major HLA loci; most
iPSCs are integrated transgene-free. This method may provide iPSCs suitable for
autologous and allologous stem-cell therapy in the future.
human induced pluripotent stem cells (iPSCs) with episomal plasmid vectors. We generated
human iPSCs from multiple donors, including two putative human leukocyte antigen (HLA)-
homozygous donors who match∼ 20% of the Japanese population at major HLA loci; most
iPSCs are integrated transgene-free. This method may provide iPSCs suitable for
autologous and allologous stem-cell therapy in the future.
Abstract
We report a simple method, using p53 suppression and nontransforming L-Myc, to generate human induced pluripotent stem cells (iPSCs) with episomal plasmid vectors. We generated human iPSCs from multiple donors, including two putative human leukocyte antigen (HLA)-homozygous donors who match ∼20% of the Japanese population at major HLA loci; most iPSCs are integrated transgene-free. This method may provide iPSCs suitable for autologous and allologous stem-cell therapy in the future.
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