Genetic studies with herpes simplex virus type 1. The isolation of temperature-sensitive mutants, their arrangement into complementation groups and recombination …
SM Brown, DA Ritchie… - Journal of General …, 1973 - microbiologyresearch.org
SM Brown, DA Ritchie, JH Subak-Sharpe
Journal of General Virology, 1973•microbiologyresearch.orgSUMMARY Nine 5-bromodeoxyuridine-induced temperature-sensitive (ts) mutants were
isolated from stocks of a syncytial plaque type mutant (syn) itself derived from the non-
syncytial wild-type (syn+) of the glasgow strain 17 of herpes simplex virus type 1. The nine ts
mutants have been assigned to eight cistrons on the basis of different complementation
tests. Non-syncytial revertants isolated from the original ts syn mutants were used in
reciprocal three-factor crosses of the type: tsX syn× tsY syn+ and tsX syn+× tsY syn. With …
isolated from stocks of a syncytial plaque type mutant (syn) itself derived from the non-
syncytial wild-type (syn+) of the glasgow strain 17 of herpes simplex virus type 1. The nine ts
mutants have been assigned to eight cistrons on the basis of different complementation
tests. Non-syncytial revertants isolated from the original ts syn mutants were used in
reciprocal three-factor crosses of the type: tsX syn× tsY syn+ and tsX syn+× tsY syn. With …
Summary
Nine 5-bromodeoxyuridine-induced temperature-sensitive (ts) mutants were isolated from stocks of a syncytial plaque type mutant (syn) itself derived from the non-syncytial wild-type (syn+) of the glasgow strain 17 of herpes simplex virus type 1. The nine ts mutants have been assigned to eight cistrons on the basis of different complementation tests.
Non-syncytial revertants isolated from the original ts syn mutants were used in reciprocal three-factor crosses of the type: tsX syn × tsY syn+ and tsX syn+ × tsY syn. With recombination data from these crosses a linkage map has been constructed. The provisional map which locates nine cistrons is linear and spans about 25 recombination units.
The regular occurrence of plaques with mixed syn/syn+ morphology in the progeny from crosses is reported.
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