[PDF][PDF] IL-27 induces the production of IgG1 by human B cells

A Boumendjel, L Tawk, R de Waal Malefijt… - European cytokine …, 2006 - researchgate.net
A Boumendjel, L Tawk, R de Waal Malefijt, V Boulay, H Yssel, J Pène
European cytokine network, 2006researchgate.net
It has been reported that IL-27 specifically induces the production of IgG2a by mouse B cells
and inhibits IL-4-induced IgG1 synthesis. Here, we show that human naïve cord blood
expresses a functional IL-27 receptor, consisting of the TCCR and gp130 subunits, although
at lower levels as compared to naïve and memory splenic B cells. IL-27 does not induce
proliferative responses and does not increase IgG1 production by CD19+CD27+ memory B
cells. However, it induces a low, but significant production of IgG1 by naïve CD19+CD27 …
Abstract
It has been reported that IL-27 specifically induces the production of IgG2a by mouse B cells and inhibits IL-4-induced IgG1 synthesis. Here, we show that human naïve cord blood expresses a functional IL-27 receptor, consisting of the TCCR and gp130 subunits, although at lower levels as compared to naïve and memory splenic B cells. IL-27 does not induce proliferative responses and does not increase IgG1 production by CD19+CD27+ memory B cells. However, it induces a low, but significant production of IgG1 by naïve CD19+CD27–IgD+IgG–spleen and cord blood B cells, activated via CD40, whereas it has no effect on the production of the other IgG subclasses. In addition, IL-27 induces the differentiation of a population of B cells that express high levels of CD38, in association with a down-regulation of surface IgD expression, and that are surface IgG+/int, CD20low, CD27high, indicating that IL-27 promotes isotype switching and plasma cell differentiation of naive B cells. However, as compared to the effects of IL-21 and IL-10, both switch factors for human IgG1 and IgG3, those of IL-27 are modest and regulate exclusively the production of IgG1. Finally, although IL-27 has no effect on IL-4 and anti-CD40-induced Ce germline promoter activity, it up-regulates IL-4-induced IgE production by naive B cells. These results point to a partial redundancy of switch factors regulating the production of IgG1 in humans, and furthermore indicate the existence of a common regulation of the human IgG1and murine IgG2a isotypes by IL-27.
researchgate.net