Seq-Well: portable, low-cost RNA sequencing of single cells at high throughput

TM Gierahn, MH Wadsworth, TK Hughes, BD Bryson… - Nature …, 2017 - nature.com
Nature methods, 2017nature.com
Single-cell RNA-seq can precisely resolve cellular states, but applying this method to low-
input samples is challenging. Here, we present Seq-Well, a portable, low-cost platform for
massively parallel single-cell RNA-seq. Barcoded mRNA capture beads and single cells are
sealed in an array of subnanoliter wells using a semipermeable membrane, enabling
efficient cell lysis and transcript capture. We use Seq-Well to profile thousands of primary
human macrophages exposed to Mycobacterium tuberculosis.
Abstract
Single-cell RNA-seq can precisely resolve cellular states, but applying this method to low-input samples is challenging. Here, we present Seq-Well, a portable, low-cost platform for massively parallel single-cell RNA-seq. Barcoded mRNA capture beads and single cells are sealed in an array of subnanoliter wells using a semipermeable membrane, enabling efficient cell lysis and transcript capture. We use Seq-Well to profile thousands of primary human macrophages exposed to Mycobacterium tuberculosis.
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