New serum factor in patients with rheumatoid arthritis: the antiperinuclear factor
RLF Nienhuis, E Mandema, C Smids - Annals of the rheumatic …, 1964 - ncbi.nlm.nih.gov
RLF Nienhuis, E Mandema, C Smids
Annals of the rheumatic diseases, 1964•ncbi.nlm.nih.govMaterials and. Methods We followed the technique described by Mandema, Pollak, Kark and
Rezaian (1961). Smears of fresh unfixed epithelial cells from healthy human buccal mucosa
were used as substrate. The labelled antiglobulin serum used was a commercial globulin
fraction of rabbit antihuman globulin serum conjugated with fluorescein isothiocyanate
(Sylvana Chemical Co.). The slides were inspected under a Reichert-Zetopan microscope
using an Osram HBO 200 mercury vapour lamp as light source.
Rezaian (1961). Smears of fresh unfixed epithelial cells from healthy human buccal mucosa
were used as substrate. The labelled antiglobulin serum used was a commercial globulin
fraction of rabbit antihuman globulin serum conjugated with fluorescein isothiocyanate
(Sylvana Chemical Co.). The slides were inspected under a Reichert-Zetopan microscope
using an Osram HBO 200 mercury vapour lamp as light source.
Materials and. Methods We followed the technique described by Mandema, Pollak, Kark and Rezaian (1961). Smears of fresh unfixed epithelial cells from healthy human buccal mucosa were used as substrate. The labelled antiglobulin serum used was a commercial globulin fraction of rabbit antihuman globulin serum conjugated with fluorescein isothiocyanate (Sylvana Chemical Co.). The slides were inspected under a Reichert-Zetopan microscope using an Osram HBO 200 mercury vapour lamp as light source.
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