[CITATION][C] Intracytoplasmic inclusions of immunoglobulins in rheumatoid arthritis and other diseases
JH Vaughan, EV Barnett, MV Sobel… - Arthritis & Rheumatism …, 1968 - Wiley Online Library
JH Vaughan, EV Barnett, MV Sobel, RF Jacox
Arthritis & Rheumatism: Official Journal of the American College …, 1968•Wiley Online LibraryMATERIALS AND METHODS Selection of patients. The peripheral blood and joint fluid
specimens examined in the present study came from patients who attended the arthritis
clinics or were hospitalized in the Strong Memorial or Rochester General Hospitals. No
particular attempt was made to select patients for this study; the joint fluids examined came
from patients who were submitted to arthrocenteses determined solely by the expediencies
of clinical care. Peripheral blood samples were drawn simultaneously on each such patient …
specimens examined in the present study came from patients who attended the arthritis
clinics or were hospitalized in the Strong Memorial or Rochester General Hospitals. No
particular attempt was made to select patients for this study; the joint fluids examined came
from patients who were submitted to arthrocenteses determined solely by the expediencies
of clinical care. Peripheral blood samples were drawn simultaneously on each such patient …
MATERIALS AND METHODS
Selection of patients. The peripheral blood and joint fluid specimens examined in the present study came from patients who attended the arthritis clinics or were hospitalized in the Strong Memorial or Rochester General Hospitals. No particular attempt was made to select patients for this study; the joint fluids examined came from patients who were submitted to arthrocenteses determined solely by the expediencies of clinical care. Peripheral blood samples were drawn simultaneously on each such patient. In addition a large and scattered number of other patients who had nonarthritic diseases provided peripheral blood specimens alone for examination. Zmmunofluorescence. All specimens for cell studies were collected in 0.01-0.02 M. EDTA. They were washed by centrifugation three times in 1 or 3 per cent bovine serum albumin in 0.15 M. NaCl. The peripheral blood leukocytes were initially isolated as the buffy coat from 1 ml. of blood spun down in a Wintrobe hematocrit tube. Staining of the cells was carried out using antisera prepared as previously described." Aggregated yC globulin was prepared from Cohn Fraction I1 protein by the protocol described by Mellors but with absorption with liver powder just before use. Cells were recorded as positive only when distinct cytoplasmic granules were seen. Diffuse cytoplasmic staining was not accepted, though sometimes noted. Other immunologic studies. Complement titers (C'HSO) were carried out using sheep RBC sensitized with 1: 1000 commercial amboceptor." By using 5 X 10'sensitized RBC in 7.0 ml. and a 40 minute incubation period at 37 C., higher CHW titers were obtained than those described in ref. 16. The latex flocculation test (LFT) for anti-yC globulins was performed by the modification described by Atwater and Jacox." Antinuclear antibodies (ANA) were detected by immunofluorescence of normal human leukocyte nuclei? 8
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