The proliferation kinetics of pulmonary alveolar macrophages

JE Coggle, JD Tarling - Journal of leukocyte biology, 1984 - academic.oup.com
JE Coggle, JD Tarling
Journal of leukocyte biology, 1984academic.oup.com
In this study the proliferation kinetics of pulmonary alveolar macrophages (PAMs) are
determined using [3H] thymidine labeling, flow cytofluorimetry, metaphase arrest, and the
percentage of labeled mitoses. The demonstration of in situ PAM proliferation is
demonstrated beyond doubt. Comparison of the turnover time, calculated from cell kinetic
parameters, with the experimentally determined migration times shows that the contribution
proliferating PAMs make to their own population size is significant. Indeed, a strong case …
Abstract
In this study the proliferation kinetics of pulmonary alveolar macrophages (PAMs) are determined using [3H]thymidine labeling, flow cytofluorimetry, metaphase arrest, and the percentage of labeled mitoses. The demonstration of in situ PAM proliferation is demonstrated beyond doubt. Comparison of the turnover time, calculated from cell kinetic parameters, with the experimentally determined migration times shows that the contribution proliferating PAMs make to their own population size is significant. Indeed, a strong case may be made to show that some 70% of the PAM population is produced by intra-alveolar divisions of “free” PAMs.
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