[HTML][HTML] Macrophages expressing arginase 1 and nitric oxide synthase 2 accumulate in the small intestine during Giardia lamblia infection
Nitric oxide (NO) has been shown to inhibit Giardia lamblia in vitro and in vivo. This study
sought to determine if Giardia infection induces arginase 1 (ARG1) expression in host
macrophages to reduce NO production. Stimulations of RAW 264.7 macrophage-like cells
with Giardia extract induced arginase activity. Real-time PCR and immunohistochemistry
showed increased ARG1 and nitric oxide synthase 2 (NOS2) expression in mouse intestine
following infection. Flow cytometry demonstrated increased numbers of macrophages …
sought to determine if Giardia infection induces arginase 1 (ARG1) expression in host
macrophages to reduce NO production. Stimulations of RAW 264.7 macrophage-like cells
with Giardia extract induced arginase activity. Real-time PCR and immunohistochemistry
showed increased ARG1 and nitric oxide synthase 2 (NOS2) expression in mouse intestine
following infection. Flow cytometry demonstrated increased numbers of macrophages …