[HTML][HTML] Dentin matrix protein 1 gene cis-regulation: use in osteocytes to characterize local responses to mechanical loading in vitro and in vivo
W Yang, Y Lu, I Kalajzic, D Guo, MA Harris… - Journal of Biological …, 2005 - ASBMB
Dentin matrix protein 1 (DMP1) is highly expressed in osteocytes and is mechanically
responsive. To study osteocyte-specific and mechanically regulated DMP1 gene expression,
the transcriptional activity of three cis-regulatory regions was first examined in an osteoblast
differentiation model in vitro using a green fluorescent protein (GFP) reporter. Expression of
the-9624 to+ 1996 bp (10 kb) and-7892 to+ 4439 bp (8 kb) DMP1 cis-regulatory regions
dramatically increased in areas of mineralized matrix, in dendritic, osteocyte-like cells …
responsive. To study osteocyte-specific and mechanically regulated DMP1 gene expression,
the transcriptional activity of three cis-regulatory regions was first examined in an osteoblast
differentiation model in vitro using a green fluorescent protein (GFP) reporter. Expression of
the-9624 to+ 1996 bp (10 kb) and-7892 to+ 4439 bp (8 kb) DMP1 cis-regulatory regions
dramatically increased in areas of mineralized matrix, in dendritic, osteocyte-like cells …