N-glycosylation of the β-propeller domain of the integrin α5 subunit is essential for α5β1 heterodimerization, expression on the cell surface, and its biological function
T Isaji, Y Sato, Y Zhao, E Miyoshi, Y Wada… - Journal of Biological …, 2006 - jbc.org
The N-glycosylation of integrin α5β1 is thought to play crucial roles in cell spreading, cell
migration, ligand binding, and dimer formation, but the underlying mechanism remains
unclear. To investigate the importance of the N-glycans of this integrin in detail, sequential
site-directed mutagenesis was carried out to remove single or combined putative N-
glycosylation sites on theα5 integrin. Removal of the putative N-glycosylation sites on the β-
propeller, Thigh, Calf-1, or Calf-2 domains of the α5 subunit resulted in a decrease in …
migration, ligand binding, and dimer formation, but the underlying mechanism remains
unclear. To investigate the importance of the N-glycans of this integrin in detail, sequential
site-directed mutagenesis was carried out to remove single or combined putative N-
glycosylation sites on theα5 integrin. Removal of the putative N-glycosylation sites on the β-
propeller, Thigh, Calf-1, or Calf-2 domains of the α5 subunit resulted in a decrease in …
