[HTML][HTML] Carbachol Stimulates Transactivation of Epidermal Growth Factor Receptor and Mitogen-activated Protein Kinase in T84Cells: IMPLICATIONS FOR …
SJ Keely, JM Uribe, KE Barrett - Journal of Biological Chemistry, 1998 - ASBMB
We have examined the role of tyrosine phosphorylation in regulation of calcium-dependent
chloride secretion across T 84 colonic epithelial cells. The calcium-mediated agonist
carbachol (CCh, 100 μm) stimulated a time-dependent increase in tyrosine phosphorylation
of a range of proteins (with molecular masses ranging up to 180 kDa) in T 84 cells. The
tyrosine kinase inhibitor, genistein (5 μm), significantly potentiated chloride secretory
responses to CCh, indicating a role for CCh-stimulated tyrosine phosphorylation in negative …
chloride secretion across T 84 colonic epithelial cells. The calcium-mediated agonist
carbachol (CCh, 100 μm) stimulated a time-dependent increase in tyrosine phosphorylation
of a range of proteins (with molecular masses ranging up to 180 kDa) in T 84 cells. The
tyrosine kinase inhibitor, genistein (5 μm), significantly potentiated chloride secretory
responses to CCh, indicating a role for CCh-stimulated tyrosine phosphorylation in negative …