Antagonistic regulation of macrophage phenotype by M-CSF and GM-CSF: implication in atherosclerosis
I Brochériou, S Maouche, H Durand, V Braunersreuther… - Atherosclerosis, 2011 - Elsevier
OBJECTIVES: We characterized the transcriptional profiles of GM-CSF-(GM-MØ) and M-CSF-
induced macrophages (M-MØ) and investigated in situ a subset of differentially expressed
genes in human and mouse atherosclerotic lesions. METHODS AND RESULTS: Using
microarrays we identified a number of genes and biological processes differentially
regulated in M-MØ vs GM-MØ. By varying in culture the M-CSF/GM-CSF ratio (0–10), a
spectrum of macrophage phenotypes was explored by RT-QPCR. M-CSF (10ng/ml) …
induced macrophages (M-MØ) and investigated in situ a subset of differentially expressed
genes in human and mouse atherosclerotic lesions. METHODS AND RESULTS: Using
microarrays we identified a number of genes and biological processes differentially
regulated in M-MØ vs GM-MØ. By varying in culture the M-CSF/GM-CSF ratio (0–10), a
spectrum of macrophage phenotypes was explored by RT-QPCR. M-CSF (10ng/ml) …
