Neurotrophins are required for nerve growth during development

KL Tucker, M Meyer, YA Barde - Nature neuroscience, 2001 - nature.com
KL Tucker, M Meyer, YA Barde
Nature neuroscience, 2001nature.com
Although the requirement of neurotrophins for the prevention of cell death in the peripheral
nervous system is well established, their physiological involvement in nerve growth is still
unclear. To address this question, we generated a mouse that expresses the green
fluorescent protein in post-mitotic neurons, allowing the repeated visualization of all motor
and sensory axons during development. We imaged the growth of these axons into the limb
bud of day 10.5 embryos. Sensory axons, but rarely motor axons, were targeted to …
Abstract
Although the requirement of neurotrophins for the prevention of cell death in the peripheral nervous system is well established, their physiological involvement in nerve growth is still unclear. To address this question, we generated a mouse that expresses the green fluorescent protein in post-mitotic neurons, allowing the repeated visualization of all motor and sensory axons during development. We imaged the growth of these axons into the limb bud of day 10.5 embryos. Sensory axons, but rarely motor axons, were targeted to ectopically placed beads containing any of the neurotrophins NGF, BDNF, NT-3 or NT-4/5. Conversely, a combination of function-blocking monoclonal antibodies to NGF, BDNF and NT-3 dramatically inhibited elongation of both sensory and motor axons in the limb bud, indicating that the growth of mixed nerves is dependent upon neurotrophins during development.
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