Radiosensitivity of patient-derived glioma stem cell 3-dimensional cultures to photon, proton, and carbon irradiation
S Chiblak, Z Tang, B Campos, Z Gal… - International Journal of …, 2016 - Elsevier
Purpose To investigate the radiosensitivity of primary glioma stem cell (GSC) cultures with
different CD133 status in a 3-dimensional (3D) model after photon versus proton versus
carbon irradiation. Methods and Materials Human primary GSC spheroid cultures were
established from tumor specimens of six consented glioblastoma patients. Human U87MG
was used as a classical glioblastoma radioresistant cell line. Cell suspensions were
generated by mechanical dissociation of GSC spheroids and embedded in a semi-solid 3D …
different CD133 status in a 3-dimensional (3D) model after photon versus proton versus
carbon irradiation. Methods and Materials Human primary GSC spheroid cultures were
established from tumor specimens of six consented glioblastoma patients. Human U87MG
was used as a classical glioblastoma radioresistant cell line. Cell suspensions were
generated by mechanical dissociation of GSC spheroids and embedded in a semi-solid 3D …