S1PR1-mediated IFNAR1 degradation modulates plasmacytoid dendritic cell interferon-α autoamplification

JR Teijaro, S Studer, N Leaf… - Proceedings of the …, 2016 - National Acad Sciences
JR Teijaro, S Studer, N Leaf, WB Kiosses, N Nguyen, K Matsuki, H Negishi, T Taniguchi…
Proceedings of the National Academy of Sciences, 2016National Acad Sciences
Blunting immunopathology without abolishing host defense is the foundation for safe and
effective modulation of infectious and autoimmune diseases. Sphingosine 1-phosphate
receptor 1 (S1PR1) agonists are effective in treating infectious and multiple autoimmune
pathologies; however, mechanisms underlying their clinical efficacy are yet to be fully
elucidated. Here, we uncover an unexpected mechanism of convergence between S1PR1
and interferon alpha receptor 1 (IFNAR1) signaling pathways. Activation of S1PR1 signaling …
Blunting immunopathology without abolishing host defense is the foundation for safe and effective modulation of infectious and autoimmune diseases. Sphingosine 1-phosphate receptor 1 (S1PR1) agonists are effective in treating infectious and multiple autoimmune pathologies; however, mechanisms underlying their clinical efficacy are yet to be fully elucidated. Here, we uncover an unexpected mechanism of convergence between S1PR1 and interferon alpha receptor 1 (IFNAR1) signaling pathways. Activation of S1PR1 signaling by pharmacological tools or endogenous ligand sphingosine-1 phosphate (S1P) inhibits type 1 IFN responses that exacerbate numerous pathogenic conditions. Mechanistically, S1PR1 selectively suppresses the type I IFN autoamplification loop in plasmacytoid dendritic cells (pDCs), a specialized DC subset, for robust type I IFN release. S1PR1 agonist suppression is pertussis toxin-resistant, but inhibited by an S1PR1 C-terminal–derived transactivating transcriptional activator (Tat)-fusion peptide that blocks receptor internalization. S1PR1 agonist treatment accelerates turnover of IFNAR1, suppresses signal transducer and activator of transcription 1 (STAT1) phosphorylation, and down-modulates total STAT1 levels, thereby inactivating the autoamplification loop. Inhibition of S1P-S1PR1 signaling in vivo using the selective antagonist Ex26 significantly elevates IFN-α production in response to CpG-A. Thus, multiple lines of evidence demonstrate that S1PR1 signaling sets the sensitivity of pDC amplification of IFN responses, thereby blunting pathogenic immune responses. These data illustrate a lipid G-protein coupled receptor (GPCR)-IFNAR1 regulatory loop that balances effective and detrimental immune responses and elevated endogenous S1PR1 signaling. This mechanism will likely be advantageous in individuals subject to a range of inflammatory conditions.
National Acad Sciences