Humanized monoclonal antibody that passively protects mice against systemic and intranasal ricin toxin challenge
G Van Slyke, EK Sully, N Bohorova… - Clinical and Vaccine …, 2016 - Am Soc Microbiol
G Van Slyke, EK Sully, N Bohorova, O Bohorov, D Kim, MH Pauly, KJ Whaley, L Zeitlin…
Clinical and Vaccine Immunology, 2016•Am Soc MicrobiolABSTRACT PB10 is a murine monoclonal antibody against an immunodominant epitope on
ricin toxin's enzymatic subunit. Here, we characterize a fully humanized version of PB10
IgG1 (hPB10) and demonstrate that it has potent in vitro and in vivo toxin-neutralizing
activities. We also report the minimum serum concentrations of hPB10 required to protect
mice against 10 times the 50% lethal dose of ricin when delivered by injection and
inhalation.
ricin toxin's enzymatic subunit. Here, we characterize a fully humanized version of PB10
IgG1 (hPB10) and demonstrate that it has potent in vitro and in vivo toxin-neutralizing
activities. We also report the minimum serum concentrations of hPB10 required to protect
mice against 10 times the 50% lethal dose of ricin when delivered by injection and
inhalation.
Abstract
PB10 is a murine monoclonal antibody against an immunodominant epitope on ricin toxin's enzymatic subunit. Here, we characterize a fully humanized version of PB10 IgG1 (hPB10) and demonstrate that it has potent in vitro and in vivo toxin-neutralizing activities. We also report the minimum serum concentrations of hPB10 required to protect mice against 10 times the 50% lethal dose of ricin when delivered by injection and inhalation.
American Society for Microbiology