[PDF][PDF] A modified colorimetric method for the estimation of N-acetylamino sugars
JL Reissig, JL Strominger, LF Leloir - 1955 - ri.conicet.gov.ar
JL Reissig, JL Strominger, LF Leloir
1955•ri.conicet.gov.arMaterials and Methods A~~ aratusOptical density of, the solution was measured with a
Beckman quartz spectrophotometer(model DU), with Corex cells and a 1 cm. light path.
Wave-length calibration was. performed with reference to the 656 rnp of the hydrogen lamp.
A further check on the calibration was afforded by the location of an absorption band at 550
rnp in a solution of cytochrome c plus sodium dithionite. Reagents Potassium tetraborate
(Fisher Scientific Company). A solution 0.8 M in borate is prepared, and the pH is adjusted to …
Beckman quartz spectrophotometer(model DU), with Corex cells and a 1 cm. light path.
Wave-length calibration was. performed with reference to the 656 rnp of the hydrogen lamp.
A further check on the calibration was afforded by the location of an absorption band at 550
rnp in a solution of cytochrome c plus sodium dithionite. Reagents Potassium tetraborate
(Fisher Scientific Company). A solution 0.8 M in borate is prepared, and the pH is adjusted to …
Materials and Methods
A~~ aratusOptical density of, the solution was measured with a Beckman quartz spectrophotometer(model DU), with Corex cells and a 1 cm. light path. Wave-length calibration was. performed with reference to the 656 rnp of the hydrogen lamp. A further check on the calibration was afforded by the location of an absorption band at 550 rnp in a solution of cytochrome c plus sodium dithionite. Reagents
Potassium tetraborate (Fisher Scientific Company). A solution 0.8 M in borate is prepared, and the pH is adjusted to 9.1 with KOH (pH 8.9 when diluted 6-fold). For many of the experiments reported here, the borate was prepared by adding the calculated amount of KOH to a H3B03 solution. The solution was then concentrated, and the crystalline mass was washed with cold water and dried. The pH was 9.2 (when diluted). p-Dimethylaminobenzaldehyde(DMAB) reagent. 10 gm. of DMAB
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