Solving the structure of macromolecular complexes using transmission electron microscopy
can be an arduous task. Many of the steps in this process rely strongly on the aid of pre-
existing structural knowledge, and are greatly complicated when this information is
unavailable. Here, we present two software tools meant to facilitate particle picking, an early
stage in the single-particle processing of unknown macromolecules. The first tool, DoG
Picker, is an efficient and reasonably general, particle picker based on the Difference of …