Intracellular staining and flow cytometry to identify lymphocyte subsets within murine aorta, kidney and lymph nodes in a model of hypertension
F Laroumanie, BL Dale, MA Saleh… - JoVE (Journal of Visualized …, 2017 - jove.com
It is now well known that T lymphocytes play a critical role in the development of several
cardiovascular diseases1, 2, 3, 4, 5. For example, studies from our group have shown that
hypertension is associated with an excessive accumulation of T cells in the vessels and
kidney during the development of experimental hypertension6. Once in these tissues, T cells
produce several cytokines that affect both vascular and renal function leading to
vasoconstriction and sodium and water retention1, 2. To fully understand how T cells cause …
cardiovascular diseases1, 2, 3, 4, 5. For example, studies from our group have shown that
hypertension is associated with an excessive accumulation of T cells in the vessels and
kidney during the development of experimental hypertension6. Once in these tissues, T cells
produce several cytokines that affect both vascular and renal function leading to
vasoconstriction and sodium and water retention1, 2. To fully understand how T cells cause …
