Flow cytometry controls, instrument setup, and the determination of positivity

HT Maecker, J Trotter - Cytometry Part A: the journal of the …, 2006 - Wiley Online Library
HT Maecker, J Trotter
Cytometry Part A: the journal of the International Society for …, 2006Wiley Online Library
A frequent goal of flow cytometric analysis is to classify cells as positive or negative for a
given marker, or to determine the precise ratio of positive to negative cells. This requires
good and reproducible instrument setup, and careful use of controls for analyzing and
interpreting the data. The type of controls to include in various kinds of flow cytometry
experiments is a matter of some debate and discussion. In this tutorial, we classify controls in
various categories, describe the options within each category, and discuss the merits of …
Abstract
A frequent goal of flow cytometric analysis is to classify cells as positive or negative for a given marker, or to determine the precise ratio of positive to negative cells. This requires good and reproducible instrument setup, and careful use of controls for analyzing and interpreting the data. The type of controls to include in various kinds of flow cytometry experiments is a matter of some debate and discussion. In this tutorial, we classify controls in various categories, describe the options within each category, and discuss the merits of each option. © 2006 International Society for Analytical Cytology
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