The ability to cryopreserve lymphocytes in peripheral blood mononuclear cells (PBMC) to
retain their function after thawing is critical to the analysis of cancer immunotherapy studies.
We evaluated a variety of cryopreservation strategies with the aim of developing an
optimized protocol for freezing and thawing PBMC to retain viability and function. We
determined several factors which do not affect cell viability after cryopreservation such as
shipping frozen samples on dry ice, the length of time and speed at which samples are …