Standardized multi-color flow cytometry and computational biomarker discovery
S Schlickeiser, M Streitz, B Sawitzki - Suppression and Regulation of …, 2016 - Springer
S Schlickeiser, M Streitz, B Sawitzki
Suppression and Regulation of Immune Responses: Methods and Protocols, Volume II, 2016•SpringerMulti-color flow cytometry has become a valuable and highly informative tool for diagnosis
and therapeutic monitoring of patients with immune deficiencies or inflammatory disorders.
However, the method complexity and error-prone conventional manual data analysis often
result in a high variability between different analysts and research laboratories. Here, we
provide strategies and guidelines aiming at a more standardized multi-color flow cytometric
staining and unsupervised data analysis for whole blood patient samples.
and therapeutic monitoring of patients with immune deficiencies or inflammatory disorders.
However, the method complexity and error-prone conventional manual data analysis often
result in a high variability between different analysts and research laboratories. Here, we
provide strategies and guidelines aiming at a more standardized multi-color flow cytometric
staining and unsupervised data analysis for whole blood patient samples.
Abstract
Multi-color flow cytometry has become a valuable and highly informative tool for diagnosis and therapeutic monitoring of patients with immune deficiencies or inflammatory disorders. However, the method complexity and error-prone conventional manual data analysis often result in a high variability between different analysts and research laboratories. Here, we provide strategies and guidelines aiming at a more standardized multi-color flow cytometric staining and unsupervised data analysis for whole blood patient samples.
Springer