Integrin alpha 7 as substrate for a glycosylphosphatidylinositol-anchored ADP-ribosyltransferase on the surface of skeletal muscle cells.
A Zolkiewska, J Moss - Journal of Biological Chemistry, 1993 - ASBMB
An arginine-specific mono-ADP-ribosyltransferase is expressed on the surface of
differentiated mouse skeletal muscle cells and is anchored in the membrane via a
glycosylphosphatidylinositol tail. Following incubation of intact cells with [adenylate-32P]
NAD and analysis by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-
PAGE), a 97-kDa [32P] ADP-ribosylated protein was observed under reducing conditions
and a 140-kDa complex under nonreducing conditions. The ADP-ribosylated protein was …
differentiated mouse skeletal muscle cells and is anchored in the membrane via a
glycosylphosphatidylinositol tail. Following incubation of intact cells with [adenylate-32P]
NAD and analysis by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-
PAGE), a 97-kDa [32P] ADP-ribosylated protein was observed under reducing conditions
and a 140-kDa complex under nonreducing conditions. The ADP-ribosylated protein was …