Sulforhodamine B (SRB) assay in cell culture to investigate cell proliferation
EA Orellana, AL Kasinski - Bio-protocol, 2016 - bio-protocol.org
The SRB assay has been used since its development in 1990 (Skehan et al., 1990) to
inexpensively conduct various screening assays to investigate cytotoxicity in cell based
studies (Vichai and Kirtikara, 2006). This method relies on the property of SRB, which binds
stoichiometrically to proteins under mild acidic conditions and then can be extracted using
basic conditions; thus, the amount of bound dye can be used as a proxy for cell mass, which
can then be extrapolated to measure cell proliferation.
inexpensively conduct various screening assays to investigate cytotoxicity in cell based
studies (Vichai and Kirtikara, 2006). This method relies on the property of SRB, which binds
stoichiometrically to proteins under mild acidic conditions and then can be extracted using
basic conditions; thus, the amount of bound dye can be used as a proxy for cell mass, which
can then be extrapolated to measure cell proliferation.