[HTML][HTML] Dynamin2, clathrin, and lipid rafts mediate endocytosis of the apical Na/K/2Cl cotransporter NKCC2 in thick ascending limbs
GR Ares, PA Ortiz - Journal of Biological Chemistry, 2012 - ASBMB
Steady-state surface levels of the apical Na/K/2Cl cotransporter NKCC2 regulate NaCl
reabsorption by epithelial cells of the renal thick ascending limb (THAL). We reported that
constitutive endocytosis of NKCC2 controls NaCl absorption in native THALs; however, the
pathways involved in NKCC2 endocytosis are unknown. We hypothesized that NKCC2
endocytosis at the apical surface depends on dynamin-2 and clathrin. Measurements of
steady-state surface NKCC2 and the rate of NKCC2 endocytosis in freshly isolated rat …
reabsorption by epithelial cells of the renal thick ascending limb (THAL). We reported that
constitutive endocytosis of NKCC2 controls NaCl absorption in native THALs; however, the
pathways involved in NKCC2 endocytosis are unknown. We hypothesized that NKCC2
endocytosis at the apical surface depends on dynamin-2 and clathrin. Measurements of
steady-state surface NKCC2 and the rate of NKCC2 endocytosis in freshly isolated rat …