LC/MS characterization of rotenone induced cardiolipin oxidation in human lymphocytes: implications for mitochondrial dysfunction associated with Parkinson's …
YY Tyurina, DE Winnica, VI Kapralova… - Molecular nutrition & …, 2013 - Wiley Online Library
YY Tyurina, DE Winnica, VI Kapralova, AA Kapralov, VA Tyurin, VE Kagan
Molecular nutrition & food research, 2013•Wiley Online LibraryScope Rotenone is a toxicant believed to contribute to the development of Parkinson's
disease. Methods and results Using human peripheral blood lymphocytes we demonstrated
that exposure to rotenone resulted in disruption of electron transport accompanied by the
production of reactive oxygen species, development of apoptosis and elevation of
peroxidase activity of mitochondria. Employing LC/MS‐based lipidomics/oxidative lipidomics
we characterized molecular species of cardiolipin (CL) and its oxidation/hydrolysis products …
disease. Methods and results Using human peripheral blood lymphocytes we demonstrated
that exposure to rotenone resulted in disruption of electron transport accompanied by the
production of reactive oxygen species, development of apoptosis and elevation of
peroxidase activity of mitochondria. Employing LC/MS‐based lipidomics/oxidative lipidomics
we characterized molecular species of cardiolipin (CL) and its oxidation/hydrolysis products …
Scope
Rotenone is a toxicant believed to contribute to the development of Parkinson's disease.
Methods and results
Using human peripheral blood lymphocytes we demonstrated that exposure to rotenone resulted in disruption of electron transport accompanied by the production of reactive oxygen species, development of apoptosis and elevation of peroxidase activity of mitochondria. Employing LC/MS‐based lipidomics/oxidative lipidomics we characterized molecular species of cardiolipin (CL) and its oxidation/hydrolysis products formed early in apoptosis and associated with the rotenone‐induced mitochondrial dysfunction.
Conclusion
The major oxidized CL species – tetra‐linoleoyl‐CL – underwent oxidation to yield epoxy‐C18:2 and dihydroxy‐C18:2 derivatives predominantly localized in sn‐1 and sn‐2 positions, respectively. In addition, accumulation of mono‐lyso‐CL species and oxygenated free C18:2 were detected in rotenone‐treated lymphocytes. These oxidation/hydrolysis products may be useful for the development of new biomarkers of mitochondrial dysfunction.
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