A method to generate enhanced GFP+ chimeric mice to study the role of bone marrow-derived cells in the eye
V Singh, R Jaini, AAM Torricelli, VK Tuohy… - Experimental eye …, 2013 - Elsevier
GFP-chimeric mice are important tools to study the role of bone marrow-derived cells in eye
physiology. A method is described to generate GFP-chimeric mice using whole-body, sub-
lethal radiation (600 rad) of wild-type C57BL/6 recipients followed by tail vein injection of
bone marrow cells derived from GFP+ (GFP-transgenic C57/BL/6-Tg (UBC-GFP) 30 Scha/J)
mice. This method yields stable GFP+ chimeras with greater than 95% chimerism (range 95–
99%), achieved within one month of bone marrow transfer confirmed by microscopy and …
physiology. A method is described to generate GFP-chimeric mice using whole-body, sub-
lethal radiation (600 rad) of wild-type C57BL/6 recipients followed by tail vein injection of
bone marrow cells derived from GFP+ (GFP-transgenic C57/BL/6-Tg (UBC-GFP) 30 Scha/J)
mice. This method yields stable GFP+ chimeras with greater than 95% chimerism (range 95–
99%), achieved within one month of bone marrow transfer confirmed by microscopy and …