Differential expression, surface binding, and response to connective tissue growth factor in lung fibroblast subpopulations
Methods and Results Primary rat lung fibroblasts and human lung fibroblasts were sorted
using antibodies to surface antigen Thy-1 or its human equivalent, CD90, into populations
of> 95% purity. Proliferation was studied in quiescent, subconfluent monolayers by
measuring incorporation of 3 H thymidine following exposure to recombinant human CTGF
(rhCTGF). In both species, Thy-1/CD90− but not Thy-1/CD90+ fibroblasts demonstrated a
concentration-dependent increase in proliferation in response to rhCTGF (Fig 1). In order to …
using antibodies to surface antigen Thy-1 or its human equivalent, CD90, into populations
of> 95% purity. Proliferation was studied in quiescent, subconfluent monolayers by
measuring incorporation of 3 H thymidine following exposure to recombinant human CTGF
(rhCTGF). In both species, Thy-1/CD90− but not Thy-1/CD90+ fibroblasts demonstrated a
concentration-dependent increase in proliferation in response to rhCTGF (Fig 1). In order to …