Differential expression, surface binding, and response to connective tissue growth factor in lung fibroblast subpopulations

JS Hagood, JA Lasky, JE Nesbitt, P Segarini - Chest, 2001 - journal.chestnet.org
Methods and Results Primary rat lung fibroblasts and human lung fibroblasts were sorted
using antibodies to surface antigen Thy-1 or its human equivalent, CD90, into populations
of> 95% purity. Proliferation was studied in quiescent, subconfluent monolayers by
measuring incorporation of 3 H thymidine following exposure to recombinant human CTGF
(rhCTGF). In both species, Thy-1/CD90− but not Thy-1/CD90+ fibroblasts demonstrated a
concentration-dependent increase in proliferation in response to rhCTGF (Fig 1). In order to …