[HTML][HTML] Molecular basis of the ligand binding specificity of αvβ8 integrin
A Ozawa, Y Sato, T Imabayashi, T Uemura… - Journal of Biological …, 2016 - ASBMB
αvβ8 is an integrin that recognizes an Arg-Gly-Asp (RGD) motif and interacts with fibronectin,
vitronectin, and latent TGF-β1. We comprehensively determined the binding activity of the
αvβ8 integrin toward 25 secreted proteins having an RGD motif. The αvβ8 integrin strongly
bound to latent TGF-β1 but showed marginal activity for other RGD-containing proteins,
including fibronectin and vitronectin. Site-directed mutagenesis of latent TGF-β1
demonstrated that the high affinity binding of αvβ8 integrin to latent TGF-β1 was defined by …
vitronectin, and latent TGF-β1. We comprehensively determined the binding activity of the
αvβ8 integrin toward 25 secreted proteins having an RGD motif. The αvβ8 integrin strongly
bound to latent TGF-β1 but showed marginal activity for other RGD-containing proteins,
including fibronectin and vitronectin. Site-directed mutagenesis of latent TGF-β1
demonstrated that the high affinity binding of αvβ8 integrin to latent TGF-β1 was defined by …