Fluorescent target array T helper assay: a multiplex flow cytometry assay to measure antigen-specific CD4+ T cell-mediated B cell help in vivo
BJC Quah, DK Wijesundara, C Ranasinghe… - Journal of …, 2013 - Elsevier
CD4+ T cells play a central role in regulating the immune response. Their effector function is
commonly assessed by their capacity to secrete cytokines detected by ELISPOT and
intracellular cytokine staining. However, one aspect of their effector function that is often
overlooked is their ability to help activation of cognate B cells directly, a process that is
initiated through the engagement of their T cell-receptor (TCR) with cognate peptide
presented on major histocompatibility complex class II (MHC-II) molecules by B cells. Here …
commonly assessed by their capacity to secrete cytokines detected by ELISPOT and
intracellular cytokine staining. However, one aspect of their effector function that is often
overlooked is their ability to help activation of cognate B cells directly, a process that is
initiated through the engagement of their T cell-receptor (TCR) with cognate peptide
presented on major histocompatibility complex class II (MHC-II) molecules by B cells. Here …