Methylglyoxal (MG), a reactive metabolic byproduct and a precursor of advanced glycation
endproducts (AGEs), is elevated in diabetes. In the body MG is free or reversibly or
irreversibly bound (mostly with proteins). Variable plasma MG values have been reported.
MG is commonly measured using high performance liquid chromatography. We tested
several protocols on different biological samples, which resulted in significant differences in
MG values measured in a given sample. The different values do not appear due to the …