Determination of D-serine and D-alanine in the tissues and physiological fluids of mice with various D-amino-acid oxidase activities using two-dimensional high …

Y Miyoshi, K Hamase, Y Tojo, M Mita, R Konno… - … of chromatography B, 2009 - Elsevier
Two-dimensional-HPLC procedures have been established for the sensitive and selective
determination of d-serine (d-Ser) and d-alanine (d-Ala), and their amounts in the tissues and
physiological fluids of mice with various d-amino-acid oxidase (DAO) activities have been
demonstrated. These two d-amino acids are modulators of the N-methyl-d-aspartate
receptor mediated neurotransmission, and the alterations in their amounts following the
changes in the DAO activity are matters of interest. After pre-column derivatization with 4 …