Positive effect of collagen V and VI on triglyceride accumulation during differentiation in cultures of bovine intramuscular adipocytes
I Nakajima, S Muroya, R Tanabe, K Chikuni - Differentiation, 2002 - Elsevier
Ethyl-3, 4-dihydroxybenzoate (EDHB), a specific inhibitor of collagen synthesis, was used to
study the role of collagen in the differentiation of bovine intramuscular preadipocytes (BIP).
Triglyceride (TG) accumulation levels of BIP cells were dose-dependently inhibited by EDHB
and were reduced to 50% at a 0.1 mM concentration. EDHB addition prevented the
accretion of collagens (types I–VI) on the cell surface, which generally increases during
adipose conversion. Western blotting and immunofluorescence studies showed in detail that …
study the role of collagen in the differentiation of bovine intramuscular preadipocytes (BIP).
Triglyceride (TG) accumulation levels of BIP cells were dose-dependently inhibited by EDHB
and were reduced to 50% at a 0.1 mM concentration. EDHB addition prevented the
accretion of collagens (types I–VI) on the cell surface, which generally increases during
adipose conversion. Western blotting and immunofluorescence studies showed in detail that …