[HTML][HTML] Biglycan and decorin bind close to the n-terminal region of the collagen VI triple helix
C Wiberg, E Hedbom, A Khairullina… - Journal of Biological …, 2001 - ASBMB
The binding of native biglycan and decorin to pepsin-extracted collagen VI from human
placenta was examined by solid phase assay and by measurement of surface plasmon
resonance in the BIAcore TM 2000 system. Both proteoglycans exhibited a strong affinity for
collagen VI with dissociation constants (KD) of∼ 30 nm. Removal of the glycosaminoglycan
chains by chondroitinase ABC digestion did not significantly affect binding. In coprecipitation
experiments, biglycan and decorin bound to collagen VI and equally competed with the …
placenta was examined by solid phase assay and by measurement of surface plasmon
resonance in the BIAcore TM 2000 system. Both proteoglycans exhibited a strong affinity for
collagen VI with dissociation constants (KD) of∼ 30 nm. Removal of the glycosaminoglycan
chains by chondroitinase ABC digestion did not significantly affect binding. In coprecipitation
experiments, biglycan and decorin bound to collagen VI and equally competed with the …
