Generally detected proteins in comparative proteomics–a matter of cellular stress response?

P Wang, FG Bouwman, ECM Mariman - Proteomics, 2009 - Wiley Online Library
P Wang, FG Bouwman, ECM Mariman
Proteomics, 2009Wiley Online Library
2-DE in combination with MS is a widely used proteomic technology platform in comparative
protein profiling studies for the identification of biomarkers for specific conditions (eg.
diseases, nutritional status and drug treatment) or components of biological processes and
pathways (eg. development, apoptosis and signal transduction). Obviously, the specificity of
such biomarker (s) or component (s) for applied study condition is crucial. We have worked
with this platform, mainly using a MALDI-TOF MS, for years and observed that some proteins …
2-DE in combination with MS is a widely used proteomic technology platform in comparative protein profiling studies for the identification of biomarkers for specific conditions (eg. diseases, nutritional status and drug treatment) or components of biological processes and pathways (eg. development, apoptosis and signal transduction). Obviously, the specificity of such biomarker (s) or component (s) for applied study condition is crucial.
We have worked with this platform, mainly using a MALDI-TOF MS, for years and observed that some proteins were often detected in different studies, regardless of the kind of study condition applied. We suspected that this phenomenon, that some proteins are generally detected, might link to the proteomic platform, ie. 2-DE in combination with MALDI-TOF MS. It greatly challenges the specificity of the outcome of each individual experiment.
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