Renewal of Paneth cells in the small intestine of the mouse
H Cheng, J Merzel, CP Leblond - American Journal of Anatomy, 1969 - Wiley Online Library
H Cheng, J Merzel, CP Leblond
American Journal of Anatomy, 1969•Wiley Online LibraryThe origin and fate of Paneth cells were examined in duodenum, jejunum and ileum of adult
female mice, using radioautography after administration of 3H‐thymidine either in a single
injection or in drinking water for four days or as a continuous infusion for up to ten days. The
tissues were fixed by perfusion with 4% paraformaldehyde. One‐micron thick,
Eponembedded single or serial sections were stained with Regaud's hematoxylin,
radioautographed, and counterstained with safranin O. Mitosis of Paneth cells is never …
female mice, using radioautography after administration of 3H‐thymidine either in a single
injection or in drinking water for four days or as a continuous infusion for up to ten days. The
tissues were fixed by perfusion with 4% paraformaldehyde. One‐micron thick,
Eponembedded single or serial sections were stained with Regaud's hematoxylin,
radioautographed, and counterstained with safranin O. Mitosis of Paneth cells is never …
Abstract
The origin and fate of Paneth cells were examined in duodenum, jejunum and ileum of adult female mice, using radioautography after administration of 3H‐thymidine either in a single injection or in drinking water for four days or as a continuous infusion for up to ten days. The tissues were fixed by perfusion with 4% paraformaldehyde. One‐micron thick, Eponembedded single or serial sections were stained with Regaud's hematoxylin, radioautographed, and counterstained with safranin O.
Mitosis of Paneth cells is never observed, nor are these cells ever labeled one hour after 3H‐thymidine. Hence, Paneth cells do not divide.
However, a few days after single injection or prolonged administration of 3H‐thymidine, labeled Paneth cells appear. The first labeled cells have tiny granules but, as the cells age, larger and larger granules are observed.
Adjacent to Paneth cells are slender undifferentiated cells which show frequent mitoses and early labeling. The evidence points to some of these cells transforming into Paneth cells. Since occasionally Paneth cells degenerate, the newly‐formed ones would provide replacement for those which die, thus insuring the steady state of the Paneth cell population. The renewal of this population is characterized by a turnover time of about three weeks.
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