Targeted gene disruption in somatic zebrafish cells using engineered TALENs
JD Sander, L Cade, C Khayter, D Reyon… - Nature …, 2011 - nature.com
JD Sander, L Cade, C Khayter, D Reyon, RT Peterson, JK Joung, JRJ Yeh
Nature biotechnology, 2011•nature.comFigure 1 Target sequences, frequencies of mutations and mutations induced by TALEns in
embryonic zebrafish cells. For each pair of TALEns, the wild-type (WT) target sequence is
shown at the top with the intended target sites of the TALEns marked in yellow. Deletions are
indicated by red dashes against a gray background, and insertions by lowercase blue letters
against a light blue background. The sizes of the insertions (+) or deletions (∆) are indicated
to the right of each mutant allele. The number of times that each mutant allele was isolated is …
embryonic zebrafish cells. For each pair of TALEns, the wild-type (WT) target sequence is
shown at the top with the intended target sites of the TALEns marked in yellow. Deletions are
indicated by red dashes against a gray background, and insertions by lowercase blue letters
against a light blue background. The sizes of the insertions (+) or deletions (∆) are indicated
to the right of each mutant allele. The number of times that each mutant allele was isolated is …
Figure 1 Target sequences, frequencies of mutations and mutations induced by TALEns in embryonic zebrafish cells. For each pair of TALEns, the wild-type (WT) target sequence is shown at the top with the intended target sites of the TALEns marked in yellow. Deletions are indicated by red dashes against a gray background, and insertions by lowercase blue letters against a light blue background. The sizes of the insertions (+) or deletions (∆) are indicated to the right of each mutant allele. The number of times that each mutant allele was isolated is shown between square brackets. Mutation frequencies are calculated as the number of mutant alleles isolated/the total number of alleles analyzed. For the hey2 gene, we also identified two larger deletions 142 and 303 bp in length, which extend substantially beyond the intended target sites of the TALEns. hey2:
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