[HTML][HTML] Covalent cross-linking of fibronectin to fibrin is required for maximal cell adhesion to a fibronectin-fibrin matrix
SA Corbett, L Lee, CL Wilson… - Journal of Biological …, 1997 - ASBMB
In a blood clot, fibrin and plasma fibronectin (pFN) are covalently cross-linked by activated
factor XIII (factor XIIIa) to form pFN-fibrin multimers. To determine the functional significance
of covalent pFN-fibrin interactions, we have developed anin vitro model which allows the
incorporation of recombinant FN (recFN) molecules into a covalently cross-linked recFN-
fibrin matrix. Using the baculovirus expression system, we have expressed recFN monomers
composed of the amino-terminal 70-kDa region and the first 11 type III repeats (WT) with …
factor XIII (factor XIIIa) to form pFN-fibrin multimers. To determine the functional significance
of covalent pFN-fibrin interactions, we have developed anin vitro model which allows the
incorporation of recombinant FN (recFN) molecules into a covalently cross-linked recFN-
fibrin matrix. Using the baculovirus expression system, we have expressed recFN monomers
composed of the amino-terminal 70-kDa region and the first 11 type III repeats (WT) with …