[HTML][HTML] Identification and quantification of protein S-nitrosation by nitrite in the mouse heart during ischemia
Abstract Nitrate (NO 3−) and nitrite (NO 2−) are known to be cardioprotective and to alter
energy metabolism in vivo. NO 3− action results from its conversion to NO 2− by salivary
bacteria, but the mechanism (s) by which NO 2− affects metabolism remains obscure. NO 2−
may act by S-nitrosating protein thiols, thereby altering protein activity. But how this occurs,
and the functional importance of S-nitrosation sites across the mammalian proteome, remain
largely uncharacterized. Here we analyzed protein thiols within mouse hearts in vivo using …
energy metabolism in vivo. NO 3− action results from its conversion to NO 2− by salivary
bacteria, but the mechanism (s) by which NO 2− affects metabolism remains obscure. NO 2−
may act by S-nitrosating protein thiols, thereby altering protein activity. But how this occurs,
and the functional importance of S-nitrosation sites across the mammalian proteome, remain
largely uncharacterized. Here we analyzed protein thiols within mouse hearts in vivo using …