Targeting MYC as a therapeutic intervention for anaplastic thyroid cancer

K Enomoto, X Zhu, S Park, L Zhao… - The Journal of …, 2017 - academic.oup.com
K Enomoto, X Zhu, S Park, L Zhao, YJ Zhu, MC Willingham, J Qi, JA Copland, P Meltzer
The Journal of Clinical Endocrinology & Metabolism, 2017academic.oup.com
Context: Recent studies showed that transcription of the MYC gene is driven by the
interaction of bromodomain and extraterminal domain (BET) proteins with acetylated
histones on chromatin. JQ1, a potent inhibitor that effectively disrupts the interaction of BET
proteins with acetylated histones, preferentially suppresses transcription of the MYC gene.
We recently reported that JQ1 decreased thyroid tumor growth and improved survival in a
mouse model of anaplastic thyroid cancer (ATC) by targeting MYC transcription. The role of …
Context
Recent studies showed that transcription of the MYC gene is driven by the interaction of bromodomain and extraterminal domain (BET) proteins with acetylated histones on chromatin. JQ1, a potent inhibitor that effectively disrupts the interaction of BET proteins with acetylated histones, preferentially suppresses transcription of the MYC gene. We recently reported that JQ1 decreased thyroid tumor growth and improved survival in a mouse model of anaplastic thyroid cancer (ATC) by targeting MYC transcription. The role of MYC in human ATC and whether JQ1 can effectively target MYC as a treatment modality have not been elucidated.
Objective
To understand the underlying molecular mechanisms of JQ1, we evaluated its efficacy in human ATC cell lines and xenograft models.
Design
We determined the effects of JQ1 on proliferation and invasion in cell lines and xenograft tumors. We identified key regulators critical for JQ1-affected proliferation and invasion of tumor cells.
Results
JQ1 markedly inhibited proliferation of four ATC cell lines by suppression of MYC and elevation of p21and p27 to decrease phosphorylated Rb and delay cell cycle progression from the G0/G1 phase to the S phase. JQ1 blocked cell invasion by attenuating epithelial-mesenchymal transition signals. These cell-based studies were further confirmed in xenograft studies in which the size and rate of tumor growth were inhibited by JQ1 via inhibition of p21-cyclin/cyclin-dependent kinase-Rb-E2F signaling.
Conclusions
These results suggest targeting of the MYC protein could be a potential treatment modality for human ATC for which effective treatment options are limited.
Oxford University Press