[HTML][HTML] Observation of mesenteric microcirculatory disturbance in rat by laser oblique scanning optical microscopy

Y Ding, Y Zhang, T Peng, Y Lu, D Jin, Q Ren, Y Liu… - Scientific Reports, 2013 - nature.com
Y Ding, Y Zhang, T Peng, Y Lu, D Jin, Q Ren, Y Liu, J Han, P Xi
Scientific Reports, 2013nature.com
Abstract Ischemia-reperfusion (I/R) injury model has been widely applied to the study of
microcirculation disturbance. In this work, we used laser oblique scanning optical
microscopy (LOSOM) to observe the microcirculation system in the mesentery of rat model.
Utilizing a localized point-scanning detection scheme, high-contrast images of leukocytes
were obtained. The extended detection capability facilitated both the automatic in vivo cell
counting and the accurate measurement of the rolling velocity of leukocytes. Statistical …
Abstract
Ischemia-reperfusion (I/R) injury model has been widely applied to the study of microcirculation disturbance. In this work, we used laser oblique scanning optical microscopy (LOSOM) to observe the microcirculation system in the mesentery of rat model. Utilizing a localized point-scanning detection scheme, high-contrast images of leukocytes were obtained. The extended detection capability facilitated both the automatic in vivo cell counting and the accurate measurement of the rolling velocity of leukocytes. Statistical analysis of the different treatment groups suggested that the distinction between I/R and sham groups with time lapse is significant.
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