Software for bead-based registration of selective plane illumination microscopy data
Nature methods, 2010•nature.com
To the Editor: Selective plane illumination microscopy (SPIM) 1 allows isotropic, time-lapse,
in toto imaging of large, living biological specimens by acquiring three-dimensional (3D)
images of the same sample from multiple angles (views). However, to realize the potential of
multiview SPIM imaging, it is necessary to reconstruct a single 3D image from the individual
views2 (Fig. 1a, Supplementary
in toto imaging of large, living biological specimens by acquiring three-dimensional (3D)
images of the same sample from multiple angles (views). However, to realize the potential of
multiview SPIM imaging, it is necessary to reconstruct a single 3D image from the individual
views2 (Fig. 1a, Supplementary
To the Editor: Selective plane illumination microscopy (SPIM) 1 allows isotropic, time-lapse, in toto imaging of large, living biological specimens by acquiring three-dimensional (3D) images of the same sample from multiple angles (views). However, to realize the potential of multiview SPIM imaging, it is necessary to reconstruct a single 3D image from the individual views2 (Fig. 1a, Supplementary
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