Orthogonal-plane fluorescence optical sectioning (OPFOS) microscopy was developed for
the purpose of making quantitative measurements of the intact mammalian cochlea and to
facilitate 3D reconstructions of complex features. A new version of this imaging apparatus
was built with a specimen chamber designed to accommodate samples as large as the
intact guinea pig bulla. This method left the cochlear connections with the vestibular system
and with the ossicles of the middle ear undisturbed, providing views within the cochlea with …