Multiview light-sheet microscope for rapid in toto imaging
U Krzic, S Gunther, TE Saunders, SJ Streichan… - Nature …, 2012 - nature.com
U Krzic, S Gunther, TE Saunders, SJ Streichan, L Hufnagel
Nature methods, 2012•nature.comWe present a multiview selective-plane illumination microscope (MuVi-SPIM), comprising
two detection and illumination objective lenses, that allows rapid in toto fluorescence
imaging of biological specimens with subcellular resolution. The fixed geometrical
arrangement of the imaging branches enables multiview data fusion in real time. The high
speed of MuVi-SPIM allows faithful tracking of nuclei and cell shape changes, which we
demonstrate through in toto imaging of the embryonic development of Drosophila …
two detection and illumination objective lenses, that allows rapid in toto fluorescence
imaging of biological specimens with subcellular resolution. The fixed geometrical
arrangement of the imaging branches enables multiview data fusion in real time. The high
speed of MuVi-SPIM allows faithful tracking of nuclei and cell shape changes, which we
demonstrate through in toto imaging of the embryonic development of Drosophila …
Abstract
We present a multiview selective-plane illumination microscope (MuVi-SPIM), comprising two detection and illumination objective lenses, that allows rapid in toto fluorescence imaging of biological specimens with subcellular resolution. The fixed geometrical arrangement of the imaging branches enables multiview data fusion in real time. The high speed of MuVi-SPIM allows faithful tracking of nuclei and cell shape changes, which we demonstrate through in toto imaging of the embryonic development of Drosophila melanogaster.
nature.com